Dr Hutch says 2-3 days.
Myra Savant says 12 hours.
This of course is regardless of the brand of extender used.
I know that Chill 5 has been tested while
left in a refrigerator and was still very good for 7-9 days. I would imagine it's possible that some girls are not compatible with it but, I send my males only with Chill 5. Many times it's sent directly to repro specialists and they have always raved about the quality. Who knows for sure?
I think I've used Chill 5 for almost two years now. Our conception success rate is very very good as it is with several breeder friends that use Minitube. I don't have a problem with any of the other extenders, in fact I used Camelot for years before this with the same successful results, and Clone before that. Chill 5 is much more convenient as someone said already.
If your semen arrives dead, or you miss you bitch consistently there may be other issues with the semen quality or fertility on your girl, or the collection procedure and exposed temperature could be an issue. Is the person doing the collecting experienced? Are they as careful as they must be? It's so easy to blame the extender.
The original question was how long it lasted in the girl, not what extender is preferred. That was answered by one poster. Are there any vets out there that can comment on the semen life "once in the bitch"?
Do they have quality control issues maybe? First time I used it to ship (as stud dog owner), we got 8 puppies. Second time, we missed and frankly, the bit I kept in my fridge looked pretty lethargic when I looked at it the next day (both shipments). Not impressed.
Am I sure it wasn't the semen? Well, with the same dog, tbe Chill5 worked (8 puppies), though the recipient said the semen could have looked better after about 4 days. Second time shipping with Chill5, with several litters in-between with same stud dog, fresh AIs, semen looked great fresh, as usual, but very lethargic after re-warming. Fridge has temp control and is kept at normal temps - not too cold.
So... makes me wonder why the same stud dog with viable fresh semen would work one time, but not the next with the same extender. Makes me think the Chill5 isn't the same each time!
BTW - did you inseminate too soon, thus the question about how long it will last in the bitch? That question has been guestimated before at 12-48 hours.