Mon at 3., wasn't tested again till today and is at 22.1. So I'm guessing she would have been at a 5.+ on tuesday and approx 12.+ on wednesday. I would have bred thurs and sat but I don't have the girl here. I would think a breeding tomorrow would be fine. The vet that did the progesterone testing and the stud owner seem to think tomorrow is too late.....thoughts ??
If it is really 22, it is too late.
Probably one of the two test is not right. I would re-test ASAP and go from there. if it over 18 again I would not breed. You are risking to have a singleton.
I knew my girl ovulated on a Monday with a progesterone of 5.9
Before we bred her on Wednesday, I had another progesterone pulled, she was 54!!! There's no rule for how quickly or how slowly they are to rise after ovulation, just that it does rise!
Bred the Wednesday and the friday and had 11 pups!
If you did the test Monday morning and it was 3.0, there is a good chance she hit 5.0+ Monday evening. Ideally you would of wanted to breed her Wednesday evening, and preferably no later than Friday evening. You might be able to get away with Saturday Morning and catch some eggs at the very end.(This is assumming that Monday's 3.0 test is accurate.)
With a progesterone of 3ng/ml on Monday my initial recommendation would have been to breed th/sat OR fri/sun. It's approximately 48 hours from LH surge to Ovulation and then another 48 hours before the ova are mature. (and it's not like ovulation is a single quick moment, it likely is happening over about 24 hours). Once the ova are mature they still remain viable for several days. The early progesterone values (initial rise and approximate ovulation) are more important than what the number is following ovulation. In this case it seems there is plenty of time, but if you are ever concerned that it is too late then a vaginal smear can easily determine if the bitch is in diestrus and it is indeed too late.
My girl had a progesterone of 2.9 on a Friday, 15.4 on Monday, we bred Tuesday early a.m., and Wednesday late afternoon and pregnancy was confirmed yesterday with 8-10. I will bet you will have a nice litter!