Hmmmm This is the second post today that said progesterone doubles every day. That is not correct. Once it hit 5.0, it goes up 5 more every day. So, after hitting 5.0, the following days would be 10, 15 and 20. Hutch ran a progesterone when he did a surgical on my girl 3 days after she hit 5.0 It was 22.0 She had 12 puppies!!!

I'm sorry to hear you missed. The one time I did a frozen a day earlier than I wanted, (the vet couldn't do it the next day) we ended up with 8 puppies. Since she missed with a surgical are you going to do a guarded culture to see if there is anything there?

I'm sorry you missed, but the timing was right on looking at the numbers.

Tiffany that is a really good idea, and I will do that, was considering that for next time anyway.
Well, from what I'm hearing from several people on the forum and others, three days after hitting 5.0 is considered good timing. I will maybe poll the forum about that in a separate post, to compare other breeders' experiences.
I really value the comments and support from those who responded---thank you, that's what this Forum is all about!
By the way, she would be due Saturday, and appears pregnant, except for no big belly. So she's in a major false pregnancy. Teats are prominent, she is tired and lovey-dovey, ravenously hungry, just no puppies!

I'd treat her as possibly pregnant just to be safe. Since she was bred and has some signs, is it possible that she has only one or a couple puppies? A friend has a champion from a bitch who was "definitely not pregnant" on relaxin tests, ultrasound, x-ray, etc. The two pups only appeared on x ray the day she was whelping! Then there was the rescue who had SEVEN puppies tucked somewhere under her rib cage invisibly. . . until we got the call from the boarding kennel who had only had her 10 days that there were two pups and more on the way. I realize that it is unlikely, but after all you put into this breeding and for her health, better safe than sorry.

We've done 3 frozen breedings total on 3 different bitch's. No misses, a small litter due to a timing delay of 12 hours we couldn't prevent and there were still 2 live puppies born. The 2 others were litters of 8 and 9 live puppies. All were non surgical, TCI's. The two most successful were done at 19ng-ml and 26ng-ml, progesterone was done 1 more time at the time of the breedings. It's not a bad idea to do 1 more at that time. For all you're spending, what is 1 more progesterone level in the grand scheme of things?

You have a good chance of the timing being right as long as she ovulated a normal amount of eggs and the semen was decent when thawed. Tuesday evening would have been a little better but it still sounds okay. It's always a smaller window of time with frozen but you sound as if you were right on the money especially doing a surgical implant (or TCI.) Do post what happens either way for others to see there is nothing wrong with some no.'s like that. Too many panic as soon as the bitch hits a 5.0 and begin breeding too early, sometimes based on a GP Vet's advice reading the information from Antech or Idexx which is incorrect. Ovulation takes place between 5.0 and 10, usually around 8.0. Not 5.0 on the nose necessarily.

Did you use Antech or Idexx out of curiousity? Idexx is known for getting faster-same day results back if notified and being a little more accurate.

If you're that worried, spend $40 + or - and call Hutch for his opinion. I think you'll be fine. Time will tell, so relax. Next time find another vet that does progesterone levels on at least Saturdays as a back up. Waiting the entire weekend is unacceptable when you're getting that close to a surgical implant with frozen semen. Jmo. Why was her next level done late on Monday instead of the morning? Again, just curious, if it should have been done on the weekend then the earlier Monday the better. You would have had results by 4 in the afternoon from morning testing instead of Tuesday morning. That's over a 12 hour difference.

P.S. I saw the thawed semen from my litter of 8, I couldn't see many live, swimming sperm. I don't know what the count was but I didn't like what I saw. Obviously, either they didn't all wake up yet or it still worked just fine anyway. Treat it like any other breeding at this point, wait and see and keep the pack away from her. Good luck.