I can think of many things people have blind spots too.

Never collect semen in a container that has been washed with detergent or water --kills sperm

Don't set your collection anywhere near sunlight

be careful to use non spermicidal lubricant…the only one I trust is from mini tube UsA

Don't use latex gloves

don't re-use ANY of the syringes/pipettes (believe it or not, I knew people who do this!)

Boy I could go on and on...

Also, if you are doing it yourself, you need to learn from someone experienced so you don't HARM your bitch doing the AI, depending on what kind of pipette you use, or catheter…and how far to insert it. Some also move it back and forth injecting the semen into both horns of the uterus

Injecting into the horns of the uterus???? LOL, are you doing a surgical implant? What kind of advice is this?

I thought exactly the same.

This is why if you have important questions, call Dr. Hutchinson for a $50 phone consult if you don't have a mentor or stud owner well versed in reproduction. You can get some good, basic ideas on here but not everyone knows everything.

This post is a perfect example of the dangers of seeking advice on a forum where anyone can post anything, even the most ridiculous information. To the uninitiated, the above advice might sound as though it was coming from an experienced, knowledgable person. Thank goodness they were not advising the dosage of worming preparation or other medication.

Thanks everyone for your replies. I am not attempting an AI on my own bitch and would only do so with proper guidance, so no worries. A friend of mine in another breed and I were discussing this situation and I was merely curious about other's expeiences. It never hurts to ask questions, otherwise, we don't learn. Thanks again!